Therefore, inhibition of some steps of the protein kinase C (PKC) transduction cascades was also discussed. Hypothesis for mechanism of action were proposed for acylphloroglucinols based on their lipophilicity and chemical structure. β-acids fraction of 125 µg/mL expressed the strongest virucidal activity (EC 50 = 15.21 µg/mL), in a drug-addition experiment on Vero cells. All hops compounds demonstrated a promising post-treatment viral inhibition, except the fraction of acylphloroglucinols, in mixture. The antiviral activity was determined by plaque reduction test and was visually confirmed by a cell-based immunofluorescence assay. For fast and efficient isolation and identification of such bioactive constituents, a silica-free countercurrent separation method was applied. The bioactive constituents of hops ( Humulus lupulus, Cannabaceae), mainly acylphloroglucinols, known as well as α- and β-acids, exerted distinct activity against CHIKV, without showing cytotoxicity. CHIKV causes chikungunya fever, which is mostly characterized by fever, arthralgia and, sometimes, a maculopapular rash. Chikungunya virus (CHIKV) is an arthropod-borne virus that belongs to the genus Alphavirus (family Togaviridae).
0 Comments
Leave a Reply. |
AuthorWrite something about yourself. No need to be fancy, just an overview. ArchivesCategories |